LR detailed

attL X attR reaction

Frame:	ACA AGT TTG TAC AAA AAA GCA GGC T------AC CCA GCT TTC TTG TAC AAA GTG GT

Entry clone	N75-ACAAGTTTGTACAAAAAAGCAGGCT------------------ACCCAGCTTTCTTGTACAAAGTGGT-N75 N75-TGTTCAAACATGTTTTTTCGTCCGA------------------TGGGTCGAAAGAACATGTTTCACCA-N75 [-----------attL1-----------] ORF [-----------attL2-----------]
	X
Destination vector	ACAAGTTTGTACAAAAAAGCAGGCT-N100--------N100-ACCCAGCTTTCTTGTACAAAGTGGT TGTTCAAACATGTTTTTTCGTCCGA-N100--------N100-TGGGTCGAAAGAACATGTTTCACCA [-----------attR1------------] ccdB [-----------attR2------------]
	=
Expression clone	ACAAGTTTGTACAAAAAAGCAGGCT------------------ACCCAGCTTTCTTGTACAAAGTGGT TGTTCAAACATGTTTTTTCGTCCGA------------------TGGGTCGAAAGAACATGTTTCACCA [--------attB1----------] ORF [--------attB2----------]
	+
By- product	N75-ACAAGTTTGTACAAAAAAGCAGGCT-N100--------N100-ACCCAGCTTTCTTGTACAAAGTGGT-N75 N75-TGTTCAAACATGTTTTTTCGTCCGA-N100--------N100-TGGGTCGAAAGAACATGTTTCACCA-N75 [-------------attP1--------------] ccdB [-------------attP2--------------]