LR reaction

  1. Assemble the reaction:
  Entry clone (50-150ng)
Destination vector
5X LR Buffer
TE pH 8.0
LR Clonase		 1-10 µl
150 ng
2 µl
to 8 µl
2 µl
  1. Incubate at 25°C for 1 hour to overnight.
  2. Add 2 µl of Proteinase K solution to each reaction. Incubate for 10 minutes at 37°C.
  3. Transform into competent cells and plate on plates containing a suitable antibiotic.