General structure of the recombination sites
attB sequences
| attB1 | ACA AGT TTG TAC AAA AAA GCA GGCT |
| attB1.1 | ACA ACT TTG TAC AAA AAA GTT G |
| attB2 | AC CCA GCT TTC TTG TAC AAA GTG GT |
| attB2.2 | -- -CA ACT TTC TTG TAC AAA GTT GT |
attB1: aca aGt ttg tac aaa aaa gCA ggct
attB1.1: acaaCtttgtacaaaaaagTTg
attB2: acCactttgtacaagaaagctgggt
attB2.2: acaactttgtacaagaaagTtg
Reading frames of the cassettes
| Reading Frame A |
|---|
| NNN ATC ACA AGT TTG TAC AAA AAA
--- --- T TTC TTG TAC AAA GTG GTG ATN NNN NNN TAG TGT TCA AAC ATG TTT TTT --- --- A AAG AAC ATG TTT CAC CAC TAN NNN |
 |
| Reading Frame B |
| NNA ATC ACA AGT TTG TAC AAA AAA
--- --- T TTC TTG TAC AAA GTG GTG ATN NNN NNT TAG TGT TCA AAC ATG TTT TTT --- --- A AAG AAC ATG TTT CAC CAC TAN NNN |
|
| Reading Frame C |
| NAT ATC ACA AGT TTG TAC AAA AAA
--- --- T TTC TTG TAC AAA GTG GTG ATN NNN NTA TAG TGT TCA AAC ATG TTT TTT --- --- A AAG AAC ATG TTT CAC CAC TAN NNN |