Per liter:
bacto-tryptone |
20 g 5 g 0.5 g 0.186 g |
Adjust to pH 7.0 with NaOH if necessary.
Just before use, add 5 ml of 2M MgCl2.
To 1L of SOB medium, add 5 ml of 40% glucose.
Per liter:
| bacto-tryptone yeast extract NaCl |
10 g |
Adjust to pH 7.0 with NaOH if necessary.